ERβ rabbit pAb

SKU: orb765179

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Description

Rabbit polyclonal antibody to ERbeta

Research Area

Product Categories/Antibodies/Primary Antibodies,Product Categories/Small Molecules,Product Categories/Antibodies

Images & Validation

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Item 1 of 4

Tested Applications	ELISA, IF, IHC-P, WB
Dilution Range	WB 1:500-2000, IF 1:50-300, IHC 1:50-300
Reactivity	Human, Mouse, Rat

Key Properties

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Clonality	Polyclonal
Isotype	IgG
Immunogen	The antiserum was produced against synthesized peptide derived from human Estrogen Receptor-beta. AA range:71-120
Molecular Weight	59
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Concentration	1 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Anti-ESR2 antibody, anti-ESTRB antibody, anti-NR3A2 antibody, anti-Estrogen receptor beta antibody, anti-ER-beta antibody, anti-Nuclear receptor subfamily 3 group A member 2 antibody

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Immunofluorescence analysis of mouse-kidney tissue. 1, ERβ Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min). 3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A + B.

Immunohistochemical analysis of paraffin-embedded Human-lung-cancer tissue. 1, ERβ Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Human-stomach-cancer tissue. 1, ERβ Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Immunohistochemical analysis of paraffin-embedded Rat-liver tissue. 1, ERβ Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.