Erk1/2 Antibody

SKU: orb166906

Description

Mouse monoclonal antibody to ERK1/2

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Cancer,Neuroscience,Signaling Pathways,Signaling Pathways/Apoptotic,Epigenetics,Infectious Diseases,Toll-Like receptor signaling;Apoptosis signaling;Interleukin signaling;Interferon-gamma signaling;Inflammation mediated by chemokine and cytokine signaling;Chemokine signaling;T cell receptor signaling;T cell activation;Fc gamma R-mediated phagocytosis;B cell receptor signaling;Crown Antibodies;Toll receptor signaling;Host-virus interaction;Viral process;Signal Transduction;Neuroscience;Immunology;Cell Biology;Cancer;Primary Antibodies

Images & Validation

• Tested Applications: FC, WB
• Dilution Range: WB: 1:1000
• Reactivity: Human, Mouse

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone No.: 784CT7.6.3
• Immunogen: Recombinant Protein
• Target: MAPK3
• Molecular Weight: 43136
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-ERK 1 antibody, anti-ERK 2 antibody, anti-ERK-2 antibody, anti-ERK1 antibody, anti-erk1/2 antibody, anti-ERK2 antibody, anti-ERT1 antibody, anti-ERT2 antibody, anti-Extracellular signal regulated kinase 1 antibody, anti-Extracellular signal-regulated kinase 2 antibody, anti-MAP kinase 1 antibody, anti-, anti-MAP kinase 2 antibody, anti-MAP kinase isoform p42 antibody, anti-MAP kinase isoform p44 antibody, anti- MAPK 1 antibody, anti-MAPK 2 antibody, anti-MAPK 3 antibody, anti-Mapk1 antibody, anti-MAPK2 antibody, anti-MAPK3 antibody, anti-Mitogen-activated protein kinase 1 antibody, anti-Mitogen-activated protein kinase 2 antibody, anti-MK01_HUMAN antibody, anti-p38 antibody, anti-p40 antibody, anti-p41 antibody, anti-p42-MAPK antibody, anti-PRKM 2 antibody

All lanes: Anti-Erk1/2 Antibody at 1:4000 dilution. Lane 1: Jurkat whole cell lysates. Lane 2: MCF-7 whole cell lysates. Lysates/proteins at 20 μg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 41 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Overlay histogram showing Jurkat cells stained (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was mouse IgG2a (1 μg/1x10^6 cells) used under the same conditions. Acquisition of >10000 events was performed.

Quick Database Links

Gene Symbol

MAPK3

UniProt

P27361

Wang, Yanqin et al. Neuroprotective Effect and Mechanism of Thiazolidinedione on Dopaminergic Neurons In Vivo and In Vitro in Parkinson's Disease PPAR Res, 2017, 4089214 (2017)

PubMed: 28356907

Qi, Lina et al. Expression patterns of claudin-5 and its related signals during luteal regression in pseudopregnant rats: The enhanced effect of additional PGF treatment Acta Histochem, 120, 221-227 (2018)

PubMed: 29449022