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Description
Research Area
Images & Validation
−| Tested Applications | IHC, WB |
|---|---|
| Dilution Range | Western Blot 1:500-1:1000 Immunofluorescence 1:100-1:200 Immunohistochemistry (Paraffin) 1:200 – 1:250 |
| Reactivity | Human, Mouse, Rabbit |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | Rabbit-IgG |
| Immunogen | Synthetic peptide / Extracellular domain corresponding to Human Estrogen Receptor alpha |
| Target | ESR1 / Estrogen receptor alpha |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Store at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative |
|---|---|
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |

Quality Guarantee
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Estrogen Receptor alpha antibody at 1/800 dilution, Lysates at 60 μg per lane, This blot was produced using a 10% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294314 overnight at 4°C.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Estrogen Receptor alpha with orb1294314 at 1/200.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Estrogen Receptor alpha with orb1294314 at 1/200.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Estrogen Receptor alpha with orb1294314 at 1/200.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Estrogen Receptor alpha with orb1294314 at 1/200.
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Protocol Information
ESR1 / Estrogen receptor alpha (orb1294314)
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