Estrogen Inducible Protein pS2 / TFF1 Antibody

SKU: orb1825361

Description

It recognizes a polypeptide of 6.5kDa, identified as pS2 estrogen-regulated protein. Its epitope is located in the c-terminus of human pS2 protein. pS2 is a trefoil peptide. Trefoil peptides are protease resistant molecules secreted throughout the gut that play a role in mucosal healing. These peptides contain three intra-chain disulfide bonds, forming the trefoil motif, or P-domain. pS2 is known to form dimers and this dimerization is thought to play a role in its protective and healing properties. About 60% of breast carcinomas are positive for pS2. Staining is cytoplasmic, often with localization to the Golgi apparatus. pS2 is shown to be localized in normal stomach mucosa, gastric fluid, goblet cells in the colon and small intestine, and in ulcerations of the gastrointestinal tract. Several studies have shown that pS2 is primarily expressed in estrogen receptor-positive breast tumors and it may define a subset of estrogen-dependent tumors that displays an increased likelihood of response to endocrine therapy.

Images & Validation

• Tested Applications: IHC-P
• Dilution Range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: Rabbit IgG, kappa
• Clone No.: TFF1/8817R
• Immunogen: A recombinant partial protein sequence (within amino acids 1-84) from the human protein was used as the immunogen for the recombinant Estrogen Inducible Protein pS2 antibody.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Aliquot the recombinant Estrogen Inducible Protein pS2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Expiration Date: 12 months from date of receipt.
• Hazard Information: This recombinant Estrogen Inducible Protein pS2 antibody is available for research use only.
• Disclaimer: For research use only

IHC staining of FFPE human colon tissue with recombinant Estrogen Inducible Protein pS2 antibody (clone TFF1/8817R). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human colon tissue with recombinant Estrogen Inducible Protein pS2 antibody (clone TFF1/8817R). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free recombinant Estrogen Inducible Protein pS2 antibody (clone TFF1/8817R) as confirmation of integrity and purity.

Quick Database Links

UniProt

P04155