Fas Antibody / CD95

SKU: orb1826219

Description

mAb rB-R18 specifically recognizes CD95, also known as Fas, a transmembrane glycoprotein with a MW of 40-45kDa, containing 8kDa of N-glycoside-linked polysaccharide. It is a receptor for TNFSF6/FASLG, a member of the nerve growth factor receptor/tumor necrosis factor superfamily, mediating receptor-triggered apoptosis. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation, which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro). CD95 antigen is expressed on the surface of various cell types, preferentially on the CD45RAlow CD45ROhigh subset of memory T lymphocytes.

Images & Validation

• Tested Applications: FACS, IF, IHC-Fr
• Dilution Range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (Frozen): 1-2ug/ml for 30 minutes at RT
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Recombinant
• Isotype: Mouse IgG2a, kappa
• Clone No.: rB-R18
• Immunogen: Recombinant human CD95 protein was used as the immunogen for the CD95 antibody.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Aliquot the CD95 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Expiration Date: 12 months from date of receipt.
• Hazard Information: This CD95 antibody is available for research use only.
• Disclaimer: For research use only

Quick Database Links

UniProt

P25445