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Description
Research Area
,Primary Antibodies;Cancer;Cell Biology;Immunology;Signal Transduction;Apoptosis;Apoptosis signaling;Allograft rejection
Images & Validation
−Item 1 of 3
| Tested Applications | IF, WB |
|---|---|
| Dilution Range | IF: 1:10~50, WB: 1:1000, WB: 1:1000 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Clone No. | RB21902 |
| Target | This FAS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 185-211 amino acids from the Central region of human FAS. |
| Molecular Weight | 37732 Da |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
|---|---|
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Tumor necrosis factor receptor superfamily member 6, Apo-1 antigen, Apoptosis-mediating surface antigen FAS, FASLG receptor, CD95, FAS, APT1, FAS1, TNFRSF6
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Western blot analysis of FAS Antibody (Center) in T47D cell line lysates (35 ug/lane).FAS (arrow) was detected using the purified Pab.
Confocal immunofluorescent analysis of FAS Antibody (Center) with T47D cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
Western blot analysis of FAS (arrow) using rabbit polyclonal FAS Antibody (Center). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the FAS gene.
Quick Database Links
Gene Symbol
This FAS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 185-211 amino acids from the Central region of human FAS.
UniProt
RefSeq (Protein):NP_000034.1, NP_690611.1, NP_690610.1
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_000034.1, NP_690611.1, NP_690610.1 |
|---|
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Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence
FAS Antibody (Center) (orb1928450)
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