Fas Ligand Antibody

SKU: orb1272552

Description

Fas Ligand Antibody

Research Area

,Apoptosis

Images & Validation

• Tested Applications: ELISA, NeA, WB
• Reactivity: Human
• Application Notes:
  Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of FasL/Apo1L (10 ng/mL), a concentration of 0.08 - 0.1 μg/mL of this antibody is required. ELISA:To detect hFasL by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hFasL. Western Blot:To detect hFasL by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hFasL is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

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Key Properties

• Antibody Type: Primary Antibody
• Clonality: Polyclonal
• Immunogen: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hFasL (human FAS Ligand).
• Target: FASLG
• Purification: Anti-hFasL specific antibody was purified by affinity chromatography employing immobilized hFasL matrix.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

APTL, FASL, CD178, CD95L, ALPS1B, CD95-L, TNFSF6, APT1LG1, Tumor necrosis factor ligand superfamily member 6, Apoptosis antigen ligand, APTL

Quick Database Links

Gene Symbol

FASLG

UniProt

P48023

RefSeq:P48023