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FBL2 Antibody

SKU: orb1247526

Description

FBL2 Antibody

Research Area

,Cancer

Images & Validation

Tested ApplicationsELISA, IHC, WB
ReactivityHuman, Rat
Predicted ReactivityBovine, Canine
Application Notes
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 48kDa band observed in Human Brain (Frontal Cortex) lysates and approx 50kDa band observed in Rat Brain lysates (calculated MW of 47.1kDa according to Human NP_036289.2, and 46.9kDa according to Rat XP_343496.4). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:In paraffin embedded Human Cerebral Cortex shows cytoplasm staining in neuronal bodies. Recommended concentration: 5-10ug/ml.

Key Properties

Antibody TypePrimary Antibody
ClonalityPolyclonal
ImmunogenThe immunogen for this antibody is: VFSNNDEGLINKK-C
TargetFBXL2
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration500 ug/mL
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

FBXL2, FBL2, F-box and leucine-rich repeat protein 2, FBL3, DKFZP564P0622, F-box protein containing leucine-rich repeats

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UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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0.1 mg
$ 880.00
Free Secondary Antibody (20 ul)0/0

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