FoxO1/3 (phospho Ser322/S325) rabbit pAb

SKU: orb768176

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Description

Rabbit polyclonal antibody to FoxO1/3 (phospho-Ser322/S325)

Research Area

Product Categories/Antibodies/Primary Antibodies,Product Categories/Small Molecules,Product Categories/Antibodies

Images & Validation

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Item 1 of 3

Tested Applications	ELISA, IHC-P, WB
Dilution Range	Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
Reactivity	Human, Mouse, Rat

Key Properties

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Clonality	Polyclonal
Isotype	IgG
Immunogen	The antiserum was produced against synthesized peptide derived from human FOXO1A/3A around the phosphorylation site of Ser322 and Ser325. AA range:291-340
Molecular Weight	97
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
Concentration	1 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Anti-FOXO1 antibody, anti-FKHR antibody, anti-FOXO1A antibody, anti-Forkhead box protein O1 antibody, anti-Forkhead box protein O1A antibody, anti-Forkhead in rhabdomyosarcoma antibody, anti-FOXO3 antibody, anti-FKHRL1 antibody, anti-FOXO3A antibody, anti-Forkhead box protein O3 antibody, anti-AF6q21 protein antibody, anti-Forkhead in rhabdomyosarcoma-like antibody

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FoxO1/3 (phospho Ser322/S325) rabbit pAb

Western Blot analysis of 3T3 cells using Phospho-FoxO1/3 (S322/S325) Polyclonal Antibody.

Western blot analysis of lysates from NIH/3T3 cells treated with Wortmannin 40nM 24h, using FOXO1A/3A (Phospho-Ser322 + Ser325) Antibody. The lane on the right is blocked with the phospho peptide.

Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).