FTL monoclonal antibody (M16), clone X1

SKU: orb2292945

Description

Mouse monoclonal antibody raised against a full length recombinant FTL.

Images & Validation

• Tested Applications: ELISA, IF, IP, WB
• Reactivity: Human

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1 Kappa
• Clone No.: X1
• Immunogen: FTL (AAH04245, 1 a.a. ~ 175 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Protein Sequence: MSSQIRQNYSTDVEAAVNSLVNLYLQASYTYLSLGFYFDRDDVALEGVSHFFRELAEEKREGYERLLKMQNQRGGRALFQDIKKPAEDEWGKTPDAMKAAMALEKKLNQALLDLHALGSARTDPHLCDFLETHFLDEEVKLIKKMGDHLTNLHRLGGPEAGLGEYLFERLTLKHD
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: In 1x PBS, pH 7.4
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Detection limit for recombinant GST tagged FTL is approximately 0.03 ng/ml as a capture antibody.

FTL monoclonal antibody (M16), clone X1 Western Blot analysis of FTL expression in K-562.

Immunofluorescence of monoclonal antibody to FTL on HeLa cell. [antibody concentration 10 ug/ml]

Immunoprecipitation of FTL transfected lysate using anti-FTL monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with FTL MaxPab rabbit polyclonal antibody.

Western Blot analysis of FTL expression in transfected 293T cell line by FTL monoclonal antibody (M16), clone X1. Lane 1: FTL transfected lysate (20 KDa). Lane 2: Non-transfected lysate.

Western blot analysis of FTL over-expressed 293 cell line, cotransfected with FTL Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with FTL monoclonal antibody (M16), clone X1 (Cat # orb2292945). GAPDH (36.1 kDa) used as specificity and loading control.

Western Blot detection against Immunogen (44.99 KDa).

Quick Database Links

RefSeq:AAH04245