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Description
Research Area
Product Categories/Antibodies/Primary Antibodies,Product Categories/Small Molecules,Product Categories/Antibodies
Images & Validation
−Item 1 of 4
| Tested Applications | ELISA, IF, IHC-P, WB |
|---|---|
| Dilution Range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications. |
| Reactivity | Human, Monkey, Mouse, Rat |
Key Properties
−| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human CXCR4 around the phosphorylation site of Ser339. AA range:303-352 |
| Molecular Weight | 38 |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Anti-CXCR4 antibody, anti-C-X-C chemokine receptor type 4 antibody, anti-CXC-R4 antibody, anti-CXCR-4 antibody, anti-FB22 antibody, anti-Fusin antibody, anti-HM89 antibody, anti-LCR1 antibody, anti-Leukocyte-derived seven transmembrane domain receptor antibody, anti-LESTR antibody, anti-NPYRL antibody, anti-Stromal cell-derived factor 1 receptor antibody, anti-SDF-1 rece antibody
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Immunofluorescence analysis of Hela cell. 1, Fusin (phospho Ser339) Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.
Western Blot analysis of HuvEc etoposide 25uM 24H cells using Phospho-Fusin (S339) Polyclonal Antibody.
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using CXCR4 (Phospho-Ser339) Antibody.
Immunofluorescence analysis of HeLa cells, using CXCR4 (Phospho-Ser339) Antibody. The picture on the right is blocked with the phospho peptide.
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Fusin (phospho Ser339) rabbit pAb (orb770434)
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