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Description
Research Area
,Recombinant-Protein
Images & Validation
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| Application Notes |
|---|
Key Properties
−| Expression System | HEK293 Cells |
|---|---|
| Biological Origin | Human |
| Biological Activity | G-CSF Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 45.4 kDa and the accession number is P09919-2. |
| Tag | N-hFc |
| Expression Region | A DNA sequence encoding the mature form of human GCSF isoform b (NP_757373.1) (Ala 30-Pro 204) was fused with the Fc region of human IgG1 at the N-terminus. Predicted N terminal: Glu 20 |
| MW | 45.4 kDa (predicted); 48 kDa (reducing condition, due to glycosylation) |
| Purity | 98.00% |
Storage & Handling
−| Storage | -20°C |
|---|---|
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
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Human G-CSF Protein, hFc Tag [orb2321318]
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 44.8 kDa after removal of the signal peptide. The apparent molecular mass of G-CSF-hFc is approximately 35-55 kDa due to glycosylation.
Mammalian
10 μg, 50 μg, 100 μg
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Protein Handling and Storage Guide
Protein Handling Guide
G-CSF Protein, Human, Recombinant (hFc) (orb1958776)
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