G6PC Antibody (Center)

SKU: orb35521

Description

Peptide affinity purified rabbit polyclonal antibody.

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Product Categories/Products/Antibodies/Primary Antibodies,Epigenetics,Primary Antibodies;Cancer;Cell Biology;Metabolism

Images & Validation

• Tested Applications: FC, IHC-P, WB
• Dilution Range: WB: 1:1000, IHC-P: 1:50-100, FACS: 1:10-50
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB30027
• Immunogen: This G6PC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 123-149 amino acids from the Central region of human G6PC
• Target: G6PC1
• Molecular Weight: 40,484 Da (Predicted band size : 40 kDa)
• Purity: This antibody is purified through a protein A column, followed by peptide affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-Glucose-6-phosphatase alpha antibody, anti-G6Pase-alpha antibody, anti-Glucose-6-phosphatase antibody, anti-G-6-Pase antibody, anti-G6Pase, G6PT antibody, anti-G6PC antibody

Western blot analysis of G6PC Antibody (Center) in K562 cell line lysates (35 ug/lane). G6PC (arrow) was detected using the purified Pab.

G6PC Antibody (Center) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Anti-G6PC Antibody (Center) at 1:1000 dilution + HepG2 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 40 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

G6PC Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the G6PC Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

Quick Database Links

Gene Symbol

G6PC1

UniProt

P35575

RefSeq (Protein):NP_001257326.1, NP_000142.2