Galectin-1 Antibody

SKU: orb1272755

Description

Galectin-1 Antibody

Research Area

,Apoptosis

Images & Validation

• Tested Applications: ELISA, WB
• Reactivity: Human
• Application Notes:
  ELISA:Indirect:To detect hGalectin-1 by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hGalectin-1.SandwichTo detect hGalectin-1 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our biotinylated Anti-Human Galectin-1as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hGalectin-1. Western Blot:To detect hGalectin-1 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hGalectin-1 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

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Key Properties

• Antibody Type: Primary Antibody
• Clonality: Polyclonal
• Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hGalectin-1. Human Galectin-1 specific antibody was purified by affinity chromatography employing immobilized hGalectin-1 matrix.
• Target: LGALS1
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

GBP, GAL1Galectin-1, 14 kDa laminin-binding protein, Gal-1

Quick Database Links

Gene Symbol

LGALS1

UniProt

P09382

RefSeq:P09382