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Galectin-1 / Human Placental Lactogen (hPL) Antibody

SKU: orb2318844

Description

Galectin-1 / Human Placental Lactogen (hPL) Antibody

Research Area

,Apoptosis|Autophagy|Cardiovascular|Immuno Oncology

Images & Validation

Tested ApplicationsELISA, IF, IHC, WB
Dilution RangeWestern Blot (1-2ug/ml); ELISA (Use Ab at 2-4ug/ml for coating) (Order Ab without BSA); Immunofluorescence (1-2ug/ml); Western Blot (1-2ug/ml); Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined.
ReactivityHuman
Application Notes
Positive Control: JEG-3, HeLa, K562 or 293 cells. Prostate, Kidney, Placenta, Stomach, Skin, Spleen, Brain or Heart.

Key Properties

HostMouse
ClonalityMonoclonal
IsotypeIgG1
Clone No.GAL1/1831
ImmunogenRecombinant fragment (around aa12-108) of human Galectin-1 protein (exact sequence is proprietary)
TargetLGALS1
Source/Expression SystemHEK293 cells
Molecular Weight14kDa
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance200ug/ml of Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
ConcentrationPurified Ab with BSA and Azide at 200ug/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

14kDa laminin-binding protein; Beta-galactoside-binding lectin L-14-I; Gal-1; GAL1; Galaptin; Galectin-1; HLBP14; HPL; Lactose-binding lectin 1; Lect14; Lectin galactoside-binding soluble 1; LGALS1; MAPK activating protein MP12; S-Lac lectin 1

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UniProt Details

No UniProt data available

NCBI Gene Details

No NCBI Gene data available

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
IF
Immunofluorescence
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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20 μg
$ 340.00
Free Secondary Antibody (20 ul)0/0

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DispatchUsually dispatched within 5-10 working days
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