Gap-43 (phospho Ser41) Antibody

SKU: orb1273526

Description

Gap-43 (phospho Ser41) Antibody

Research Area

,Homeostasis,Neuroscience,Phospho-Specific

Images & Validation

• Tested Applications: WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  In some tissues the antibody also recognizes a higher molecular weight band that is also recognized by the Pan GAP-43 antibody and this band may be a GAP-43 aggregate or oligomer. Immunolabeling of the GAP-43 protein in Western blots of rat brain is blocked by the Ser41 phosphopeptide used as antigen but not by the corresponding non-phosphopeptide. Note: the GAP-43 protein migrates on SDS-PAGE as a 50 kDa band. The antibody is purified by sequential chromatography on phospho- and non-phosphopeptide affinity columns. Antibody dilutions and tissue load should be based on tissue type and expected phosphorylation state. Initial recommended range of dilutions: 1:500 to 1:2000. Applications include Dot blots (DB) and Western blots (WB). Immunohistochemistry (IHC) application has not yet been determined. Human, mouse and rat have 100% amino acid sequence identity with for the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.

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Key Properties

• Antibody Type: Primary Antibody
• Clonality: Polyclonal
• Immunogen: GAP-43 (Ser41) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho-Ser41 of rat GAP-43.
• Target: Gap43
• Molecular Weight: 50
• Purification: Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

Gene Symbol

Gap43

UniProt

P07936

RefSeq:P07936