GAPDH Antibody, HRP Conjugate

SKU: orb1926019

Description

Purified Mouse Monoclonal Antibody (Mab)

Research Area

,Crown Antibodies;Primary Antibodies;Antibody Collections;Cancer;Metabolism;Microbiology;Neuroscience;Signal Transduction;Stem Cells;Loading Control

Images & Validation

• Tested Applications: WB
• Dilution Range: WB: 1:40000, WB: 1:1000
• Reactivity: Human

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: 1653CT401.3.33
• Target: This GAPDH antibody is generated from a mouse immunized with a recombinant protein of human GAPDH.
• Molecular Weight: 36053 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified monoclonal antibody conjugated with HRP supplied in PBS with 0.05% (W/V) Proclin 300. This antibody is purified through a protein G column, followed by size-exclusion - Membrane filtration purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

GAPD

Anti-GAPDH Antibody, HRP Conjugate at 1:40000 dilution + Hela whole cell lysate. Lysates/proteins at 20 µg per lane. Predicted band size: 36 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-GAPDH Antibody (conjugated HRP) at 1:1000 dilution. Lane 1: NIH/3T3 whole cell lysate. Lane 1: mouse brain lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Mouse IgG, (H+L), Peroxidase conjugated at 1/15000 dilution.Observed band size: 36kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

This GAPDH antibody is generated from a mouse immunized with a recombinant protein of human GAPDH.

UniProt

P04406