GAPDH Antibody Loading Control

SKU: orb248123

Description

Due to its ubiquitous presence in mammalian cells, GAPDH is often used a loading control protein in western blot. GAPDH antibody is used to control for/demonstrate equivalent protein loading in different gel lanes, typically transfected vs mock or non transfected cell lysate.

Research Area

Product Categories/Products/Antibodies,Product Categories/Products/Antibodies/Primary Antibodies

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: Western blot: 0.5-1ug/ml,IHC (FFPE): 3-5ug/ml
• Reactivity: Canine, Human, Mouse, Primate, Rat
• Application Notes:
  Provided assay concentrations are suggestions only, GAPDH antibody titration may be required for optimal results.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: GAP259
• Immunogen: A recombinant protein fragment from C-terminal GAPDH was used as the immunogen for the GAPDH antibody.
• Purity: Protein G Affinity Chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Aliquot and store the loading control antibody frozen at -20°C or colder to avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: 1X PBS, pH 7.4
• Expiration Date: 12 months from date of receipt.
• Hazard Information: This GAPDH antibody loading control is available for research use only.
• Disclaimer: For research use only

Western blot testing of human samples with GAPDH antibody at 0.5 ug/ml.

Western blot testing of mouse samples with GAPDH antibody at 0.5 ug/ml.

Western blot testing of human/mouse samples with GAPDH antibody at 0.5 ug/ml.

IHC testing of FFPE human liver tissue with GAPDH antibody at 3 ug/ml.

Quick Database Links

NCBI Gene ID

2597