Queen's Award Received in 2021 ISO 9001 Certified Delivered over 1,000,000 bio-reagents to life science researchers Trusted by Life Science Communities
Cart summary

You have no items in your shopping cart.

GAPDH Antibody

SKU: orb1247971

Description

GAPDH Antibody

Research Area

,Homeostasis

Images & Validation

Tested ApplicationsELISA, IF, IHC, WB
ReactivityHuman, Mouse, Rat
Predicted ReactivityCanine
Application Notes
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 37kDa band observed in Human Liver, Testis and Tonsil, and in Mouse Liver and Rat Heart lysates, and in cell lysates of HeLa and NIH3T3 (calculated MW of 36.1kDa according to Human NP_002037.2, Mouse NP_032110.1, and Rat NP_058704.1). Recommended concentration: 0.03-0.1ug/ml. Primary incubation 1 hour at room temperature. This product has been successfully used in WB on Human: PMID: 29953622 and PMID: 29158223.Immunohistochemistry:In paraffin embedded Human Liver shows textured cytoplasm staining in hepatocytes. Recommended concentration: 2ug/ml.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U251 and HeLa cells. Recommended concentration: 5-10ug/ml.

Key Properties

Antibody TypePrimary Antibody
ClonalityPolyclonal
ImmunogenThe immunogen for this antibody is: C-GVNHEKYDNSLK
TargetGAPDH
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration500 ug/mL
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

GAPDH, glyceraldehyde-3-phosphate dehydrogenase, HGNC:4141, G3PD, GAPD, MGC88685, OTTHUMP00000174431, OTTHUMP00000174432, aging-associated gene 9 protein, glyceraldehyde 3-phosphate dehydrogenase

Similar Products

  • GAPDH Mouse Monoclonal Antibody (Loading Control) [orb500708]

    WB

    Canine, Gallus, Monkey, Porcine, Rabbit, Sheep

    Hamster, Human, Mouse, Rat

    Mouse

    Monoclonal

    Unconjugated

    200 μg, 50 μl, 100 μl, 500 μl
  • GAPDH Rabbit Polyclonal Antibody (Loading Control) [orb500826]

    ELISA,  IF,  IHC-Fr,  IHC-P,  WB

    Mouse, Rat

    Hamster, Human, Mouse, Rat

    Rabbit

    Polyclonal

    Unconjugated

    50 μl, 100 μl, 500 μl
  • GAPDH Recombinant Rabbit Monoclonal Antibody (Loading Control) [orb704534]

    ICC,  IF,  IHC-Fr,  IHC-P,  WB

    Gallus, Monkey, Zebrafish

    Human, Mouse, Rat

    Rabbit

    Recombinant

    Unconjugated

    500 μl, 100 μl, 50 μl
  • GAPDH antibody [orb555879]

    ICC,  IHC-P,  IP,  WB

    Bacteria, Bovine, Canine, Drosophila, E. coli, Fish, Gallus, Hamster, Human, Insect, Mammal, Monkey, Mouse, Other, Plant, Porcine, Rabbit, Rat, Yeast, Zebrafish

    Rabbit

    Polyclonal

    Unconjugated

    100 μl
  • GAPDH Antibody [orb238246]

    ELISA,  IF,  IHC,  WB

    Human

    Rabbit

    Polyclonal

    Unconjugated

    50 μg, 100 μg
Quality Guarantee

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
IF
Immunofluorescence
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

Select a size below

0.1 mg
$ 880.00
Free Secondary Antibody (20 ul)0/0

Please add an antibody product to your cart first.

DispatchUsually dispatched within 5-10 working days
Bulk Enquiry