GAPDH

SKU: orb1294414

Description

Rabbit polyclonal antibody to GAPDH

Research Area

,Product Categories/Antibodies,Loading control

Images & Validation

• Tested Applications: IF, IHC, WB
• Dilution Range: Western Blot 1:5000-1:10000 Immunofluorescence 1:200-1:500 Immunohistochemistry (Paraffin) 1:400-1:600
• Reactivity: Human, Mouse, Rabbit

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit-IgG
• Immunogen: Synthetic peptide / encompassing a sequence within the C-terminus region.
• Target: GAPDH
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C 100mM Tris Glycine, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Anti-GAPDH antibody - 1/5000 dilution, Lysates at 20 μg per lane. This blot was produced using a 10% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294414 overnight at 4°C.

Anti-GAPDH antibody - 1/5000 dilution, Lysates at 20 μg per lane. This blot was produced using a 10% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294414 overnight at 4°C.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294414 anti-GAPDH antibody (red) at 1:200 and 4°C.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294414 anti-GAPDH antibody (red) at 1:200 and 4°C.

Immunohistochemical analysis of paraffin embedded mouse testis labeling GAPDH with orb1294414 at 1/400.

Quick Database Links

Gene Symbol

GAPDH