GCV

SKU: orb81138

Description

Ganciclovir (GCV) is a pro-drug nucleoside analog that is activated by phosphorylation. It is useful in the study of gene therapy in cancer research. Ganciclovir is a synthetic analogue of 2'-deoxy-guanosine. It is initially phosphorylated to a deoxyguanosine triphosphate (dGTP) analogue. This mechanism competitively inhibits the integration of dGTP by viral DNA polymerase, resulting in the termination of elongation of viral DNA. Upon expression of a viral suicide gene encoding thymidine kinase, the non-toxic pro-drug is converted to a phosphorylated active analog and is incorporated into the DNA of replicating eukaryotic cells, causing death of the malignant dividing cell. The cell cycle is irreversibly arrested at the G2-M checkpoint. Gap junction involvement in the ganciclovir bystander effect has been studied. Ganciclovir has been used to study loss of telomeres and to evaluate sensitivity of viruses to antiviral treatments. Ganciclovir is used in molecular biology for selection against random recombination events when homologous recombination of a gene of interest is required. Ganciclovir is a white to off-white crystalline powder with a molecular formula of C9H13N5O4 and a molecular weight of 255.23.

Research Area

,Product Categories/Proteins

Images & Validation

• Application Notes:
  Recombinant & Natural Proteins

Key Properties

• Purity: Greater than 99.0%.

Storage & Handling

• Storage: Stability: Lyophilized Ganciclovir although stable at room temperature for 3 weeks, should be stored at 4°C
• Form/Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Expiration Date: 6 months from date of receipt.
• Disclaimer: For research use only