General Heparin (Hep) ELISA Kit

SKU: orb782056

Description

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Heparin(Hep) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Heparin(Hep).

Research Area

Protein Biochemistry

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 1000 ng/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Hep protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Hep. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hep in the samples is then determined by comparing the OD of the samples to the standard curve

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Key Properties

• Reactivity: All
• Sample Types: serum, plasma and other biological fluids
• Assay Type: Competitive
• Assay Time: 2.5h
• Range: 15.63-1000 ng/mL
• Sensitivity: 4.63 ng/mL
• Concentration: 1000 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Expiration Date: Please enquire.
• Disclaimer: For research use only

Alternative Names

Unfractionated heparin ELISA Kit