GFAP Antibody

SKU: orb1939391

Description

Mouse Monoclonal Antibody (Mab)

Research Area

,Primary Antibodies;Neuroscience;Signal Transduction

Images & Validation

• Tested Applications: IF, IHC-P, WB
• Dilution Range: IF: 1:10~50, WB: 1:4000, WB: 1:500~1000, IHC-P-Leica: 1:1000, IHC: 1:2000
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b,k
• Clone No.: 183CT3.1.5
• Target: This GFAP monoclonal antibody is generated from mouse immunized with GFAP recombinant protein.
• Molecular Weight: 49880 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Glial fibrillary acidic protein, GFAP, GFAP

All lanes: Anti-GFAP Antibody at 1:4000 dilution. Lane 1: human brain lysate. Lane 2: human cerebellum lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 50 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Confocal immunofluorescent analysis of GFAP Antibody with brain tissue followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).

GFAP Antibody western blot analysis in MCF-7 cell line lysates (35 μg/lane). This demonstrates the GFAP antibody detected the GFAP protein (arrow).

Immunohistochemical analysis of paraffin-embedded Human brain section using Pink1. diluted at 1:2000 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded human brain tissue performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature; antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:1000) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary Antibody.

Quick Database Links

Gene Symbol

This GFAP monoclonal antibody is generated from mouse immunized with GFAP recombinant protein.

UniProt

P14136

RefSeq (Protein):NP_001229305.1, NP_002046.1, NP_001124491.1