GNAS Antibody (C-term)

SKU: orb31105

Description

Rabbit polyclonal antibody to YE016 belongs to the tubulin family, which is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.

Research Area

Product Categories/Products/Antibodies,Product Categories/Research Area,Product Categories/Products/Antibodies/Primary Antibodies,Epigenetics,Primary Antibodies

Images & Validation

• Tested Applications: FC, IF, IHC-P, WB
• Dilution Range: WB: 1:1000, IHC-P: 1:10-50, IF/ICC: 1:10-50, FACS: 1:10-50
• Reactivity: Human
• Predicted Reactivity: Hamster, Mouse, Porcine, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB33001
• Immunogen: Synthetic Peptide
• Target: GNAS
• Molecular Weight: 44250
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-GNAS antibody

Western blot analysis of GNAS (arrow) using rabbit polyclonal GNAS Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GNAS gene.

Confocal immunofluorescent analysis of GNAS Antibody (C-term) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

GNAS Antibody (C-term) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

GNAS Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of GNAS Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

GNAS Antibody (C-term) western blot analysis in 293, NCI-H292 cell line lysates (35 ug/lane). This demonstrates the GNAS antibody detected the GNAS protein (arrow).

Quick Database Links

Gene Symbol

GNAS

UniProt

Q5FWY2