Goat anti-AIM2 Antibody

SKU: orb20475

Description

Goat polyclonal antibody to AIM2

Research Area

Apoptotic, Cancer, Immunology, Neuroscience, Signaling Pathways

Images & Validation

• Tested Applications: ELISA, FC, IF, IHC, WB
• Dilution Range: ELISA: 1:8000, WB: 1-3 μg/ml, IHC-P: 4-6µg/ml
• Reactivity: Human
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Preliminary experiments gave an approx. 80kDa band in Human Peripheral Blood Mononucleocytes lysates after 1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 39.0kDa according to NP_004824.1. The 80kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: AIM2
• Protein Sequence: GEKLQLTSGVHSTIK
• Molecular Weight: 39.0; 26.8
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti absent in melanoma 2 antibody, anti PYHIN4 antibody, anti AIM2 antibody

Immunohistochemical staining of Human Spleen using AIM2 antibody

Western blot analysis of WI-38 lysate using AIM2 antibody

Quick Database Links

Gene Symbol

AIM2

NCBI Gene ID

9447