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ATF2 Antibody

SKU: orb18311

Description

Goat polyclonal antibody to ATF2

Research Area

Cancer, DNA Damage, Epigenetics

Images & Validation

Tested ApplicationsELISA, WB
Dilution RangeELISA: 1:32000, WB: 0.3-1 μg/ml
ReactivityHuman
Predicted ReactivityBovine, Canine, Rat
Application Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Experiments gave bands at approx 55kDa and 30kDa in lysates of Human Placenta and of cell line HeLa after 0.5 μg/ml antibody staining. These bands correspond to earlier findings in literature with different antibodies (Bailey et al , J Clin Endocrinol Metab. 2002 Apr;87(4):1717-28.; PMID: 11932306). This protein has a calculated MW of 54.5kDa according to NP_001871.2. Recommended concentration: 0.5-1.5 μg/ml.

Key Properties

HostGoat
ClonalityPolyclonal
TargetATF2
Protein SequenceIVMAPSSQSQPSGS
Molecular Weight54.5
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

anti ATF2 antibody, anti activating transcription factor 2 antibody, anti HB16 antibody, anti CREB2 antibody, anti TREB7 antibody, anti CRE-BP1 antibody, anti cAMP responsive element binding protein 2 antibody, anti MGC111558 antibody, anti activating transcription factor 2 splice variant ATF2-var2 antibody, anti cAMP responsive element binding protein 2, formerly antibody

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NCBI Gene Details

No NCBI Gene data available

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

Select a size below

100 μg
$ 500.00
Choose Conjugation or Carrier Free Version
Free Secondary Antibody (20 ul)0/0

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