ATF6 (aa437-449) Antibody

SKU: orb107677

Description

Goat polyclonal antibody to ATF6

Images & Validation

• Tested Applications: ELISA, FC, IF, WB
• Dilution Range: ELISA: 1:128000, WB: 0.3-2 μg/ml, IHC-P: 10ug/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Porcine, Rat
• Application Notes:
  WB: Approx 75-80kDa band observed in lysates of cell lines HeLa and MCF7, and in HeLa and Jurkat nuclear cell lysates (calculated MW of 74.6kDa according to NP_031374.2). Recommended concentration: 0.3-2µg/ml. Primary incubation 1 hour at room temperature.. Notes: Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 and HeLa cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration:

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: ATF6 (aa437-449)
• Protein Sequence: NSYRYDHSVSNDK
• Molecular Weight: 74.6
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti-Activating transcription factor 6 alpha antibody, anti-Activating transcription factor 6 antibody, anti-ATF 6 antibody, anti-ATF6 alpha antibody, anti-ATF6 antibody, anti-ATF6-alpha antibody, anti-ATF6A antibody, anti-cAMP dependent transcription factor ATF 6 alpha antibody, anti-ESTM49 antibody, anti-FLJ21663 antibody, anti-Processed cyclic AMP dependent transcription factor ATF 6 alpha antibody

Western blot analysis of HeLa (Lane 1) and MCF7 (Lane 2) cell lysate, and HeLa (Lane 3) and Jurkat (Lane 4) nuclear cell lysate using ATF6 antibody

Immunofluorescence analysis of A431 cells, permeabilized with 0.15% Triton of ATF6 antibody

Immunofluorescence analysis of HeLa cells, permeabilized with 0.15% Triton of ATF6 antibody

Flow cytometric analysis of A431 cells using ATF6 antibody

Quick Database Links

Gene Symbol

ATF6 (aa437-449)

NCBI Gene ID

22926