ATP5A1 Antibody

SKU: orb178380

Description

Goat polyclonal antibody to ATP5A1

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:128000, WB: 0.01-0.03 μg/ml
• Reactivity: Human, Mouse, Porcine, Rat
• Predicted Reactivity: Bovine, Canine
• Application Notes:
  WB: Approx 55kDa band observed in Human, Mouse, Rat and Pig Heart lysates (calculated MW of 54.5kDa according to NP_001001935.1). Recommended concentration: 0.01-0.03µg/ml.. Notes: Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: ATP5A1
• Protein Sequence: RVHGLRNVQAEE
• Molecular Weight: 59.8; 57.5; 54.5
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle antibody, anti ATP5A antibody, anti ATP5AL2 antibody, anti ATPM antibody, anti MOM2 antibody, anti hATP1 antibody, anti ATP synthase alpha chain, mitochondrial antibody, anti ATP synthase subunit alpha, mitochondrial antibody, anti ATP synthase, H+ transportin antibody

Quick Database Links

Gene Symbol

ATP5A1

NCBI Gene ID

498

Sikorski, Krzysztof et al. A high-throughput pipeline for validation of antibodies Nat Methods, 15, 909-912 (2018)

PubMed: 30377371

Applications

WB