B7-H4 Antibody

SKU: orb19600

Description

Goat polyclonal antibody to VTCN1

Research Area

Epigenetics, Immunology

Images & Validation

• Tested Applications: ELISA, FC, IF, WB
• Dilution Range: ELISA: 1:64000, WB: 0.1-1 μg/ml, IHC-P: 10ug/ml
• Reactivity: Human
• Predicted Reactivity: Canine, Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx. 60kDa band observed in Human Lung, Spleen and Thymus lysates (calculated MW of 30.9kDa according to NP_078902.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Simon et al, Cancer Res. 2006 Feb 1;66(3):1570-5.; PMID: 16452214). In transfected HEK293 transiently expressing B7-H4 the band of approx. 60kDa is also observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3-1 μg/ml.

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Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: B7-H4
• Protein Sequence: SKGKGNANLEYK
• Molecular Weight: 30.9; 20.7
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti B7-H4 antibody, anti B7H4 antibody, anti B7S1 antibody, anti B7X antibody, anti B7h.5 antibody, anti FLJ22418 antibody, anti PRO1291 antibody, anti RP11-229A19.4 antibody, anti VCTN1 antibody, anti T cell costimulatory molecule B7x antibody, anti immune costimulatory protein antibody, anti V-set domain containing T cell activation inhibitor 1 antibody

Quick Database Links

Gene Symbol

B7-H4

NCBI Gene ID

79679