calbindin D28 Antibody

SKU: orb12289

Description

Goat Polyclonal antibody to Calbindin

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:128000, WB: 0.03-0.1 μg/ml, IHC-P: 5 μg/ml
• Reactivity: Human, Rat
• Predicted Reactivity: Bovine, Canine, Porcine
• Application Notes:
  WB: Approx. 26kDa band observed in Human and Rat Kidney lysates and in Rat Brain lysates (calculated MW of 30.0kDa according to Human NP_004920.1and Rat NP_114190.1). The observed molecular weight is routinely observed by other sources. Recommended concentrat. IHC: Paraffin embedded Human Kidney. Recommended concentration: 5-7µg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: calbindin D28
• Protein Sequence: KTFVDQYGQRDDGK
• Molecular Weight: 30
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CALB1; calbindin 1, 28kDa; CALB; D-28K; OTTHUMP00000166027; OTTHUMP00000225441; RTVL-H protein; calbindin; calbindin 1, (28kD); calbindin D28; vitamin D-dependent calcium-binding protein, avian-type

Quick Database Links

Gene Symbol

calbindin D28

NCBI Gene ID

793