Goat anti-CARD6 Antibody

SKU: orb18496

Description

Goat polyclonal antibody to CARD6

Research Area

Apoptotic, Cancer, Epigenetics, Neuroscience, Signaling Pathways

Images & Validation

• Tested Applications: ELISA, IHC
• Dilution Range: ELISA: 1:32000, IHC-P: 5-10µg/ml
• Reactivity: Human
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Pancreas shows variable staining of cytoplasm in acinar cells. Recommended concentration, 5-10ug/ml.WB: Approx. 45-50kDa and 25-30kDa bands observed in A549, Human Heart and Human Skeletal Muscle lysates at 1ug of primary. Please note that the bands we observe are different from the predicted size of approx. 119kDa according to NP_115976. Currently we cannot find an explanation for this in the literature. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any splice variants/modified forms been reported

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Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: CARD6
• Protein Sequence: ATESTPSEIIERER
• Molecular Weight: 116
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti CARD6 antibody, anti caspase recruitment domain family, member 6 antibody, anti caspase recruitment domain protein 6 antibody, anti CINCIN1 antibody, anti CARD-containing inhibitor of Nod1 and Cardiak-induced NF-kB activation antibody

Quick Database Links

Gene Symbol

CARD6

NCBI Gene ID

84674