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Caveolin 1 Antibody

SKU: orb19043

Description

Goat polyclonal antibody to Caveolin 1

Images & Validation

Tested ApplicationsELISA, FC, IF, IHC, WB
Dilution RangeELISA: 1:8000, WB: 0.001-0.01 μg/ml, IHC-P: 2.5μg/ml, FACS: 10ug/ml, IF/ICC: 10μg/ml.
ReactivityHuman
Predicted ReactivityCanine, Mouse, Porcine, Rat
Application Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 21kDa and 25kDa observed in Human Heart, Lung and Spleen lysates. These bands correspond to earlier findings in literature with different antibodies (Wiechen et al Am J Pathol. 2001 Mar;158(3):833-9, PMID: 11238032 ). In transfected HEK293 transiently expressing Caveolin 1 a band of approx. 23kDa is observed. This band is not observed in the non-transfected HEK293. This protein has a calculated MW of 20.5kDa according to NP_001744.2. Recommended concentration: 0.3-1 μg/ml.

Key Properties

HostGoat
ClonalityPolyclonal
TargetCaveolin 1
Protein SequenceDELSEKQVYDAH
Molecular Weight20.5; 17.0
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

anti BSCL3 antibody; anti CAV 1 antibody; anti CAV antibody; anti CAV1 antibody; anti Caveolin-1 antibody; anti Caveolin1 antibody; anti cell growth-inhibiting protein 32 antibody; anti CGL3 antibody; anti LCCNS antibody

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NCBI Gene Details

No NCBI Gene data available

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
FC
Flow Cytometry
View Protocol
IF
Immunofluorescence
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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100 μg
$ 500.00
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Free Secondary Antibody (20 ul)0/0

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