CCKBR Antibody

SKU: orb19186

Description

Goat polyclonal antibody to Gastrin Receptor

Research Area

Cancer, Epigenetics, GPCR, Neuroscience, Signaling Pathways

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:16000, WB: 1-3 μg/ml, IHC-P: 6 μg/ml
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Pancreas, shows strong staining in islets of Langerhans and weak staining in acinar cells. Recommended concentration, 3ug/ml.WB: Approx. 75kDa band observed in purified membranes of Rat insulinoma cell line RIN14B (calculated MW of 48.4kDa according to NP_033957.1). Data were published by El-Kouhen K and Morisset J, Int J Endocrinol. 2009;2009:875167; PMID: 20011057. The observed molecular weight also corresponds to earlier findings in literature with different antibodies (Morisset et al J Histochem Cytochem. 2003 Nov;51(11):1501-13; PMID: 14566022). Recommended concentration: 0.1-0.3 μg/ml.

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Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: CCKBR
• Protein Sequence: FDGDSDSDSQSRVRNQ
• Molecular Weight: 48.4
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-G14 antibody, anti-G17 antibody, anti-G34 antibody, anti-G52 antibody, anti-G6 antibody, anti-GAS antibody, anti-Gast antibody, anti-GAST_HUMAN antibody, anti-Gastrin antibody, anti-Gastrin component I antibody, anti-Gastrin component II antibody, anti-Gastrin component III antibody, anti-Gastrin precursor antibody, anti-Gastrin-14 antibody, anti-GASR_HUMAN antibody,anti-GASR antibody, anti-Gastrin receptor antibody

Quick Database Links

Gene Symbol

CCKBR

NCBI Gene ID

887