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Description
Research Area
Epigenetics, Immunology
Images & Validation
−| Tested Applications | ELISA, FC, IF, WB |
|---|---|
| Dilution Range | ELISA: 1:128000, WB: 0.01-0.1 μg/ml, IF/ICC: 10μg/ml, FACS: 10ug/ml |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Host | Goat |
|---|---|
| Clonality | Polyclonal |
| Target | CD274 / PD-L1 |
| Protein Sequence | CKKQSDTHLEET |
| Molecular Weight | 33.3; 20.2 |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
|---|---|
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Anti-CD274 antibody, anti-Programmed cell death 1 ligand 1 antibody, anti-Programmed death ligand 1 antibody, anti-B7 homolog 1 antibody, anti-B7-H1 antibody.

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Protocol Information
WB
Western Blot (IB, immunoblot)
FC
Flow Cytometry
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)




