Goat anti-Chat (mouse) Antibody

SKU: orb19261

Description

Goat polyclonal antibody to Chat

Research Area

Neuroscience

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:128000, WB: 0.3-1 μg/ml, IHC-P: 1:2000
• Reactivity: Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 75-80kDa band observed in Mouse and Rat Brain lysates (calculated MW of 72.3kDa according to NP_034021.1). Recommended concentration: 0.2-0.5 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: Chat (mouse)
• Protein Sequence: KERARGPTKPSNLD
• Molecular Weight: 72.3
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti-Acetyl CoA choline O acetyltransferase antibody, anti- Acetyl CoA:choline O acetyltransferase antibody, anti- ChAT antibody, anti- CHOACTase antibody, anti- Choline acetylase antibody, anti- choline acetyltransferase antibody, anti- Choline O acetyltransferase antibody, anti- Choline O-acetyltransferase antibody, anti- CLAT_HUMAN antibody, anti- CMS1A antibody, anti- CMS1A2 antibody, anti- EC 2.3.1.6 antibody, anti- OTTHUMP00000019583 antibody, anti- OTTHUMP00000019584 antibody

orb19261 (0.2ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemical staining of Mouse Brain lysate using Chat antibody

Quick Database Links

Gene Symbol

Chat (mouse)