Clusterin/APOJ Antibody

SKU: orb19080

Description

Goat polyclonal antibody to CLU

Research Area

Apoptotic, Cancer, Epigenetics, Immunology, Neuroscience, Signaling Pathways

Images & Validation

• Tested Applications: ELISA, IF, IHC, WB
• Dilution Range: ELISA: 1:64000, WB: 0.03-0.1 μg/ml, IHC-P: 2.5 μg/ml
• Reactivity: Human
• Predicted Reactivity: Canine
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 35-37kDa band observed in human colon, kidney and prostate lysates (calculated MW of 57.8kDa according to NP_NP_001822.2 and 52.5 according to NP_976084.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (PMID: 9713325 and PMID: 11560857). Recommended concentration: 0.3-1.0 μg/ml.

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Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: Clusterin / APOJ
• Protein Sequence: EKALQEYRKKHREE
• Molecular Weight: 57.8; 52.5
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti Clusterin antibody, anti CLU antibody, anti APOJ antibody, anti CLI antibody, anti KUB1 antibody, anti Apolipoprotein J antibody, anti Apo-J antibody

Quick Database Links

Gene Symbol

Clusterin / APOJ

NCBI Gene ID

1191