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CYBB/GP91-PHOX Antibody

SKU: orb20254

Description

Goat polyclonal to CYBB

Research Area

Epigenetics

Images & Validation

Tested ApplicationsELISA, IHC
Dilution RangeELISA: 1:64000, IHC-P: 1-2µg/ml
ReactivityHuman
Predicted ReactivityCanine
Application Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:2000.WB: Approx 85Da band observed in Human Peripheral Blood Mononucleocytes ysates (calculated MW of 65.3kDa according to NP_000388.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Teahan et al, Nature. 1987 Jun 25-Jul 1;327(6124):720-1. PMID: 3600769). Recommended concentration: 0.03-0.1 μg/ml.

Key Properties

HostGoat
ClonalityPolyclonal
TargetCYBB / GP91-PHOX
Protein SequenceSYLNFARKRIKNP
Molecular Weight65.3
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

anti CYBB antibody, anti cytochrome b-245, beta polypeptide (chronic granulomatous disease) antibody, anti CGD antibody, anti GP91-1 antibody, anti GP91-PHOX antibody, anti GP91PHOX antibody, anti NOX2 antibody, anti cytochrome b-245, beta polypeptide antibody
Quality Guarantee

Quality Guarantee

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NCBI Gene Details

No NCBI Gene data available

Protocol Information

IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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100 μg
$ 500.00
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Free Secondary Antibody (20 ul)0/0

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