DEAD-box protein 6 Antibody

SKU: orb19176

Description

Goat polyclonal antibody to DDX6

Research Area

Cancer, Epigenetics

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:128000, WB: 0.3-1 μg/ml, IHC-P: 5 μg/ml
• Reactivity: Human
• Predicted Reactivity: Canine, Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 50kDa band observed in lysates of cell line Jurkat (calculated MW of 53.2kDa according to NP_NP_004388.1). In transfected HEK293 transiently expressing Human DDX6 a band of approx. 55kDa is observed. This band is not observed in the non-transfected HEK293. The endogenous 53kDa protein is observed in both. Recommended concentration: 0.1-0.3 μg/ml.

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Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: DEAD-box protein 6
• Protein Sequence: AEYHSEPVEDEKP
• Molecular Weight: 53.2
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti DEAD-box protien 6 antibody, anti DEAD (Asp-Glu-Ala-Asp) box polypeptide 6 antibody, anti DDX6 antibody, anti HLR2 antibody, anti P54 antibody, anti RCK antibody, anti DEAD box-6 antibody, anti DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 6 (RNA helicase, 54kD) antibody

Quick Database Links

Gene Symbol

DEAD-box protein 6

NCBI Gene ID

1656