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Description
Images & Validation
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Item 1 of 3
| Tested Applications | ELISA, IF, IHC |
|---|---|
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:128000. IHC: In paraffin embedded Human breast cancer shows cell membrane and cytoplasm staining in the affected cells of Her+ type and not in triple negative type. Recommended concentration: 4-6µg/ml. Immunofluorescence: Strong expression of the protein seen in U2OS cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of U2OS cells. Recommended concentration: 10ug/ml. |
| Reactivity | Human |
Key Properties
−| Host | Goat |
|---|---|
| Clonality | Polyclonal |
| Target | ERBB2 / HER2 |
| Protein Sequence | PREYVNARHCLP |
| Molecular Weight | 138; 135 |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−anti-CD340 antibody, anti- ERBB2 antibody, anti- HER 2 antibody, anti- HER 2/neu antibody, anti- HER2 antibody, anti- MLN 19 antibody, anti- MLN19 antibody, anti- NEU antibody, anti- NGL antibody, anti- p185 antibody, anti- p185erbB2 antibody, anti- Proto oncogene c ErbB 2 antibody, anti- Proto oncogene Neu antibody, anti- TKR1 antibody
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Protocol Information
IHC
Immunohistochemistry
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Available Sizes
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Free Secondary Antibody (20 ul)0/0
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Usually dispatched within 5-10 working days