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Description
Research Area
Apoptotic, Cancer, Epigenetics, Neuroscience, Signaling Pathways
Images & Validation
−Item 1 of 2
| Tested Applications | ELISA, FC, WB |
|---|---|
| Dilution Range | ELISA: 1:16000, WB: 0.1-0.3 μg/ml, IHC-P: 10ug/ml |
| Reactivity | Human |
| Predicted Reactivity | Bovine, Canine, Mouse, Porcine, Rat |
| Application Notes |
Key Properties
−| Host | Goat |
|---|---|
| Clonality | Polyclonal |
| Target | ERN1 / IRE1a |
| Protein Sequence | PNAHGKIKAMISD |
| Molecular Weight | 110 |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−anti ERN1 antibody, anti IRE1 antibody, anti endoplasmic reticulum to nucleus signaling 1 antibody, anti FLJ30999 antibody, anti IRE1P antibody, anti MGC163277 antibody, anti MGC163279 antibody, anti ER to nucleus signalling 1 antibody, anti endoplasmic reticulum to nucleus signalling 1 antibody, anti inositol-requiring 1 antibody, anti protein kinase/endoribonuclease antibody, anti IRE1a, IRE1-alpha antibody

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Western blot analysis of Human Pancreas lysate using ERN1 antibody.

Flow cytometric analysis of HeLa cells using ERN1 antibody.
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
FC
Flow Cytometry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
ERN1/IRE1a Antibody (orb20044)
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