ERN2/IRE1b Antibody

SKU: orb19821

Description

Goat polyclonal antibody to ERN2

Research Area

Apoptotic, Cancer, Epigenetics, Neuroscience, Signaling Pathways

Images & Validation

• Tested Applications: ELISA
• Dilution Range: ELISA: 1:4000, WB: 0.3 μg/ml
• Predicted Reactivity: Human
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:4000.WB: Preliminary experiments gave an approx 37kDa band in Human Duodenum, Pancreas and Placenta lysates after 0.3 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 107kDa according to NP_150296.3. The 37kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: ERN2 / IRE1b
• Protein Sequence: QKRLQSPSKQAQPLD
• Molecular Weight: 107
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti ERN2 antibody, anti endoplasmic reticulum to nucleus signaling 2 antibody, anti IRE1b antibody, anti ER to nucleus signalling 2 antibody, anti IRE1 beta antibody, anti IRE1, S. cerevisiae, homolog of antibody, anti endoplasmic reticulum to nucleus signalling 2 antibody, anti inositol-requiring 1 beta antibody

Quick Database Links

Gene Symbol

ERN2 / IRE1b

NCBI Gene ID

10595