Flotillin 1 Antibody

SKU: orb18696

Description

Goat polyclonal to Flotillin 1

Research Area

Epigenetics

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:32000, WB: 0.1-0.3 μg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Porcine
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 48kDa band observed in lysates of cell line H460 (calculated MW of 47.4kDa according to NP_005794.1). In transfected HEK293 transiently expressing FLOT1 a band of approx. 48kDa is observed. This band is not observed in the non-transfected HEK293. Recommended for use at 0.3-1 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: Flotillin 1
• Protein Sequence: SISQVNHKPLRTA
• Molecular Weight: 47.4; 42.1
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti FLOT1 antibody, anti flotillin 1 antibody, anti reggie-2 antibody, anti integral membrane component of caveolae antibody, anti DADB-118P11.2 antibody

Western blot analysis of H460 lysate using Flotillin 1 antibody

Western blot analysis of HEK293 lysate using Flotillin 1 antibody

Immunohistochemical staining of Human Kidney using Flotillin 1 antibody

Quick Database Links

Gene Symbol

Flotillin 1

NCBI Gene ID

10211