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GAD2/GAD65 Antibody

SKU: orb18998

Description

Goat polyclonal antibody to GAD65

Research Area

Epigenetics, Neuroscience

Images & Validation

Tested ApplicationsELISA, IHC, WB
Dilution RangeELISA: 1:32000, WB: 0.5-1.5 μg/ml
ReactivityHuman
Predicted ReactivityCanine, Mouse, Rat
Application Notes
This antibody is expected to recognise both reported variants.ELISA: antibody detection limit dilution 1:16000.WB: Approx 65kDa band observed in Human Brain (Cerebral Cortex, Cerebellum and Hippocampus) lysates (calculated MW of 65.4kDa according to NP_000809.1). Recommended concentration: 0.3-1µg/ml.

Key Properties

HostGoat
ClonalityPolyclonal
TargetGAD2 / GAD65
Protein SequenceTLEDNEERMSRLSK
Molecular Weight65.4; 65.4
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

anti-65 kDa glutamic acid decarboxylase antibody, anti-DCE 2 antibody, anti-DCE2 antibody, anti-DCE2_HUMAN antibody, anti-GAD 2 antibody, anti-GAD 65 antibody, anti-GAD-2 antibody, anti-GAD-65 antibody, anti-Gad2 antibody, anti-Glutamate decarboxylase 2 (pancreas) antibody, anti-Glutamate Decarboxylase 2 (pancreatic islets and brain 65kDa) antibody, anti-Glutamate decarboxylase 2 antibody, anti-Glutamate Decarboxylase 65 antibody, anti-Glutamate decarboxylase 65 kDa isoform antibody, anti-Glutamic Acid Decarboxylase 2 antibody, anti-Glutamic Acid Decarboxylase 65 antibody, anti-MGC161605 antibody, anti-MGC161607 antibody

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Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

NCBI Gene Details

No NCBI Gene data available

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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100 μg
$ 500.00
Choose Conjugation or Carrier Free Version
Free Secondary Antibody (20 ul)0/0

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