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GAPDH (Internal) Antibody

SKU: orb19168

Description

Goat polyclonal antibody to GAPDH

Research Area

Apoptotic, Cancer, Epigenetics, Neuroscience, Signaling Pathways

Images & Validation

Tested ApplicationsELISA, IF, IHC, WB
Dilution RangeELISA: 1:16000, WB: 0.03-0.1 μg/ml, IHC-P: 2 μg/ml
ReactivityHuman, Mouse, Rat
Predicted ReactivityCanine
Application Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:4000.WB: Approx 36kDa band observed in Human Liver, Testes and Tonsil lysates and in Mouse Spleen lysates (calculated MW of 36.1kDa according to NP_002037.2). Recommended concentration: 0.1-0.3 μg/ml.

Key Properties

HostGoat
ClonalityPolyclonal
TargetGAPDH (Internal)
Protein SequenceGVNHEKYDNSLK
Molecular Weight36.1
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

anti GAPDH antibody, anti glyceraldehyde-3-phosphate dehydrogenase antibody, anti HGNC:4141 antibody, anti G3PD antibody, anti GAPD antibody, anti MGC88685 antibody, anti OTTHUMP00000174431 antibody, anti OTTHUMP00000174432 antibody, anti aging-associated gene 9 protein antibody, anti glyceraldehyde 3-phosphate dehydrogenase antibody

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Quality Guarantee

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NCBI Gene Details

No NCBI Gene data available

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
IF
Immunofluorescence
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

Select a size below

100 μg
$ 500.00
Choose Conjugation or Carrier Free Version
Free Secondary Antibody (20 ul)0/0

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