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Homeobox A5 Antibody

SKU: orb19677

Description

Goat polyclonal antibody to HOXA5

Images & Validation

Tested ApplicationsELISA
Dilution RangeELISA: 1:16000, WB: 0.5 μg/ml
Predicted ReactivityHuman, Mouse
Application Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Preliminary experiments gave an approx 100kDa band in Human Breast and Heart lysates after 0.5 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 29.3kDa according to NP_061975.2. The 100kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

HostGoat
ClonalityPolyclonal
TargetHomeobox A5
Protein SequenceDSHHGGKNSLSN
Molecular Weight29.3
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

anti HOXA5 antibody, anti homeobox A5 antibody, anti HOX1 antibody, anti HOX1.3 antibody, anti HOX1C antibody, anti MGC9376 antibody, anti homeo box 1C antibody, anti homeo box A5 antibody, anti homeobox protein HOXA5 antibody

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NCBI Gene Details

No NCBI Gene data available

Protocol Information

ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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