HSPA5/GRP78 Antibody

SKU: orb334058

Description

Goat polyclonal antibody to HSPA5

Images & Validation

• Tested Applications: ELISA, IF, IHC, WB
• Dilution Range: ELISA: 1:16000, WB: 0.3-1 μg/ml, IHC-P: 5 μg/ml
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: Approx 75kDa band observed in lysates of cell lines HepG2 and MCF7 and in Mouse Liver lysates, and approx. 80kDa band in Mouse Testis and Rat Brain lysates (calculated MW of 72.3kDa according to Human NP_005338.1, Mouse NP_071705.3 and Rat NP_037215.1). . IHC: Paraffin embedded Human Brain (Cortex). Recommended concentration: 5µg/ml.. Notes: Immunofluorescence: Expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 5µg/ml

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: HSPA5 / GRP78
• Protein Sequence: ESHQDADIEDFK
• Molecular Weight: 72.3
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

HSPA5, heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa), BIP, GRP78, HEL-S-89n, MIF2, 78 kDa glucose-regulated protein, endoplasmic reticulum lumenal Ca(2+)-binding protein grp78, epididymis secretory sperm binding protein Li 89n, immunoglobu

Western blot analysis of HepG2 (Lane 1) and MCF7 (Lane 2) lysates using HSPA5 antibody

Western blot analysis of Mouse Liver (Lane 1) and Testes (Lane 2) and Rat Brain (Lane 3) lysate using HSPA5 antibody

Immunofluorescence analysis of HeLa cells using HSPA5 antibody

Quick Database Links

Gene Symbol

HSPA5 / GRP78

NCBI Gene ID

3309