IGF1 Antibody

SKU: orb125044

Description

Goat Polyclonal to IGF1. NP_001104753.1; NP_001104754.1; NP_001104755.1; NP_000609.1, PMID: 20230795 and GeneID: 3479.

Images & Validation

• Tested Applications: ELISA, FC, IF, IHC, WB
• Dilution Range: ELISA: 1:32000, WB: 0.5-2 μg/ml, IHC-P: 3.75 μg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Rat
• Application Notes:
  WB: Approx 15kDa band observed in Human Uterus lysates (calculated MW of 15.2kDa according to NP_001104754.1). Recommended concentration 0.5-2µg/ml. Primary incubation 1 hour at room temperature. Negative Control: HepG2 cell lysate. Prelimi. IHC: Paraffin embedded Human Spleen. Recommended concentration: 3.75µg/ml.. Notes: Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HEK293 cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10ug/ml

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Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: IGF1
• Protein Sequence: RSVRAQRHTD
• Molecular Weight: 17.8; 15.2; 21.8; 17.0
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti IGF1 antibody, anti insulin-like growth factor 1 (somatomedin C) antibody, anti IGF1A antibody, anti IGFI antibody, anti OTTHUMP00000195084 antibody, anti insulin-like growth factor 1 antibody

Quick Database Links

Gene Symbol

IGF1

NCBI Gene ID

3479

Wang, Yan et al. Rumen-Protected Glucose Stimulates the Insulin-Like Growth Factor System and mTOR/AKT Pathway in the Endometrium of Early Postpartum Dairy Cows Animals (Basel), 10, E357 (2020)

PubMed: 32102173