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MAD2L1 Antibody

SKU: orb18705

Description

Goat polyclonal antibody to MAD2L1

Research Area

Epigenetics

Images & Validation

Tested ApplicationsELISA, WB
Dilution RangeELISA: 1:32000, WB: 2-4 μg/ml
ReactivityHuman
Predicted ReactivityBovine, Canine, Mouse, Porcine, Rat
Application Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 24kDa band observed in lysates of cell line HEK293 (calculated MW of 23.5kDa according to NP_002349.1). Recommended concentration: 2-4 μg/ml. An additional band of unknown identity was also consistently observed at approx. 29kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Key Properties

HostGoat
ClonalityPolyclonal
TargetMAD2L1
Protein SequenceKVNSMVAYKIPVND
Molecular Weight23.5
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

anti MAD2L1 antibody, anti MAD2 mitotic arrest deficient-like 1 (yeast) antibody, anti MAD2 antibody, anti HSMAD2 antibody, anti mitotic arrest deficient, yeast, homolog-like 1 antibody, anti MAD2 (mitotic arrest deficient, yeast, homolog)-like 1 antibody, anti MAD2-like 1 antibody, anti MAD2-like protein 1 antibody

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NCBI Gene Details

No NCBI Gene data available

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Available Sizes

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100 μg
$ 500.00
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Free Secondary Antibody (20 ul)0/0

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