MBD2 (isoform 1) Antibody

SKU: orb19388

Description

Goat polyclonal antibody to MBD2

Research Area

Epigenetics

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:64000, WB: 0.3-1 μg/ml, IHC-P: 2.5 μg/ml
• Reactivity: Human
• Predicted Reactivity: Canine, Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.IHC: In paraffin embedded Human Colon shows nuclear stianing of enterocytes. Recommended concentration, 2-4ug/ml.WB: Approx 48-50kDa band observed in lysates of cell line Jurkat (calculated MW of 43.3kDa according to NP_003918.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Tan et al, Mol Cell Biol. 2006 Oct;26(19):7224-35.; PMID: 16980624). Recommended concentration: 0.3-1 μg/ml.

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Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: MBD2 (isoform 1)
• Protein Sequence: RNDPLNQNKGKPDLN
• Molecular Weight: 43.3; 31.7
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti MBD2 antibody, anti methyl-CpG binding domain protein 2 antibody, anti DKFZp586O0821 antibody, anti DMTase antibody, anti NY-CO-41 antibody

Quick Database Links

Gene Symbol

MBD2 (isoform 1)

NCBI Gene ID

8932