NCKAP1 Antibody

SKU: orb44874

Description

Goat polyclonal antibody to NCKAP1

Images & Validation

• Tested Applications: ELISA, FC, IF
• Dilution Range: ELISA: 1:128000, WB: 0.1 μg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Rat
• Application Notes:
  WB: Preliminary experiments gave an approx 100kDa band in Human Cerebellum, Cerebral Cortex, Skin and Tonsil lysates after 0.5µg/ml antibody staining, and was successfully blocked by incubation with the immunizing peptide. Please note the calculated MWt.is 12. Notes: Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U2OS and A431 cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration:

  Read more...

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: NCKAP1
• Protein Sequence: TPREYLTSHLEIR
• Molecular Weight: 129; 130
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 0.5 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti FLJ11291 antibody, anti HEM2 antibody, anti KIAA0587 antibody, anti membrane-associated protein HEM-2 antibody, anti MGC8981 antibody, anti NAP 1 antibody, anti NAP1 antibody, anti NAP125 antibody, anti NCKAP1 antibody, anti NCK-associated protein 1 antibody, anti OTTHUMP00000163460 antibody, anti OTTHUMP00000163461 antibody, anti p125Nap1 antibody

Quick Database Links

Gene Symbol

NCKAP1

NCBI Gene ID

10787